diff --git a/misc/qutebrowser.spec b/misc/qutebrowser.spec
index e12e9a9b0..d8e1dbd25 100644
--- a/misc/qutebrowser.spec
+++ b/misc/qutebrowser.spec
@@ -82,7 +82,9 @@ def get_data_files():
 
 
 def get_hidden_imports():
-    imports = ['PyQt5.QtOpenGL', 'PyQt5._QOpenGLFunctions_2_0']
+    # FIXME:qt6 double check if those are needed
+    ver = "6" if "PYINSTALLER_QT6" in os.environ else "5"
+    imports = [f'PyQt{ver}.QtOpenGL', f'PyQt{ver}._QOpenGLFunctions_2_0']
     for info in loader.walk_components():
         imports.append(info.name)
     return imports
diff --git a/scripts/dev/build_release.py b/scripts/dev/build_release.py
index eb8a24249..3cf074086 100755
--- a/scripts/dev/build_release.py
+++ b/scripts/dev/build_release.py
@@ -330,7 +330,7 @@ def build_mac(
                         gh_token=gh_token)
 
     utils.print_title("Building .app via pyinstaller")
-    call_tox('pyinstaller-64', '-r', debug=debug)
+    call_tox(f'pyinstaller-64{"-qt6" if qt6 else ""}', '-r', debug=debug)
     utils.print_title("Patching .app")
     patch_mac_app(qt6=qt6)
     utils.print_title("Re-signing .app")
@@ -399,6 +399,7 @@ def _get_windows_python_path(x64: bool) -> pathlib.Path:
 
 def _build_windows_single(
     *, x64: bool,
+    qt6: bool,
     skip_packaging: bool,
     debug: bool,
 ) -> List[Artifact]:
@@ -412,7 +413,10 @@ def _build_windows_single(
     _maybe_remove(out_path)
 
     python = _get_windows_python_path(x64=x64)
-    call_tox(f'pyinstaller-{"64" if x64 else "32"}', '-r', python=python, debug=debug)
+    suffix = "64" if x64 else "32"
+    if qt6:
+        suffix += "-qt6"
+    call_tox(f'pyinstaller-{suffix}', '-r', python=python, debug=debug)
 
     out_pyinstaller = dist_path / "qutebrowser"
     shutil.move(out_pyinstaller, out_path)
@@ -464,12 +468,14 @@ def build_windows(
             x64=True,
             skip_packaging=skip_packaging,
             debug=debug,
+            qt6=qt6,
         )
     if not only_64bit and not qt6:
         artifacts += _build_windows_single(
             x64=False,
             skip_packaging=skip_packaging,
             debug=debug,
+            qt6=qt6,
         )
 
     return artifacts
diff --git a/tox.ini b/tox.ini
index fb66a7308..2f9b198e5 100644
--- a/tox.ini
+++ b/tox.ini
@@ -156,13 +156,16 @@ commands =
     {envpython} scripts/dev/check_doc_changes.py {posargs}
     {envpython} scripts/asciidoc2html.py {posargs}
 
-[testenv:pyinstaller-{64,32}]
+[testenv:pyinstaller-{64,32}{,-qt6}]
 basepython = {env:PYTHON:python3}
 passenv = APPDATA HOME PYINSTALLER_DEBUG
+setenv =
+    qt6: PYINSTALLER_QT6=true
 deps =
     -r{toxinidir}/requirements.txt
     -r{toxinidir}/misc/requirements/requirements-pyinstaller.txt
-    -r{toxinidir}/misc/requirements/requirements-pyqt.txt
+    !qt6: -r{toxinidir}/misc/requirements/requirements-pyqt.txt
+    qt6: -r{toxinidir}/misc/requirements/requirements-pyqt-6.txt
 commands =
     {envbindir}/pyinstaller --noconfirm misc/qutebrowser.spec