myVar equ $20

LDA #3
STA myVar
LDA #0
STA myVar+1  ;equivalent C: uint16_t myVar = 3;

negate:
LDA myVar+1
EOR #255
STA myVar+1

LDA myVar
EOR #255
STA myVar
CLC
ADC #1
STA myVar
;this handles the case if we started with something where the low byte was zero.
LDA myVar+1
ADC #0
STA myVar+1